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KMID : 0545120140240121685
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 12 p.1685 ~ p.1689
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Enhanced Production of ε-Caprolactone by Coexpression of Bacterial Hemoglobin Gene in Recombinant Escherichia coli Expressing Cyclohexanone Monooxygenase Gene
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Lee Won-Heong
Park Eun-Hee
Kim Myoung-Dong
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Abstract
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Baeyer-Villiger (BV) oxidation of cyclohexanone to ε-caprolactone in a microbial system expressing cyclohexanone monooxygenase (CHMO) can be influenced by not only the efficient regeneration of NADPH but also a sufficient supply of oxygen. In this study, the bacterial hemoglobin gene from Vitreoscilla stercoraria (vhb) was introduced into the recombinant Escherichia coli expressing CHMO to investigate the effects of an oxygen-carrying protein on microbial BV oxidation of cyclohexanone. Coexpression of Vhb allowed the recombinant E. coli strain to produce a maximum ε-caprolactone concentration of 15.7 g/l in a fed-batch BV oxidation of cyclohexanone, which corresponded to a 43% improvement compared with the control strain expressing CHMO only under the same conditions.
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KEYWORD
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Cyclohexanone monooxygenase, ε-caprolactone, bacterial hemoglobin, Escherichia coli
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